Consumers use different hair care products to change the physical appearance of their hair,\nsuch as shampoos, conditioners, hair dye and hair straighteners. They expect cosmetics products to\nbe available in the market to meet their needs in a broad and effective manner. Evaluating efficacy of\nhair care products in vitro involves the use of highly accurate equipment. This review aims to discuss\nin vitro methodologies used to evaluate the effects of hair care products on hair fiber, which can\nbe assessed by various methods, such as Scanning Electron Microscopy, Transmission Electron\nMicroscopy, Atomic Force Microscopy, Optical Coherence Tomography, Infrared Spectroscopy, Raman\nSpectroscopy, Protein Loss, Electrophoresis, color and brightness, thermal analysis and measuring\nmechanical resistance to combing and elasticity. The methodology used to test hair fibers must be\nselected according to the property being evaluated, such as sensory characteristics, determination\nof brightness, resistance to rupture, elasticity and integrity of hair strain and cortex, among others.\nIf equipment is appropriate and accurate, reproducibility and ease of employment of the analytical\nmethodology will be possible. Normally, the data set must be discussed in order to obtain conclusive\nanswers to the test.
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